Biotechnology for Young Scientists
With the Biotechnology package for Young Scientists you can study several biotechnology topics.
You will also be introduced to the concept of electrophoresis with this complete, safe, economical and easy to use package.
Isolate and observe DNA from onions.
Describe the appearance and physical characteristics of isolated DNA.
Understand the principles and method of electrophoresis.
Demonstrate the use of electrophoresis.
Recognize the advantages and uses of biotechnology and discuss its risks and benefits.
Includes teacher’s manual and worksheets and students (in English) as well as all the necessary materials.
The SET contains enough materials for 6 groups of students. (class of 40 students)
For electrophoresis you will need 5x 9V Batteries.
The kit includes:
125 mL of ethyl alcohol
40 test tubes
40 DNA vaccination tubes
40 centrifuge tubes
2 g of agarose
30 g of cell lysis solution
30 mL Concentrated blue cell label
1 battery-powered gel electrophoresis
125 mL TBE 10X buffer
Set of 6 samples of electrophoresis paints
2 large onions
Cell lysis solution
Dissolve the cell lysate powder in 500mL of deionized water and stir until the solution is clear.
Dilute 10mL of 10X buffer with 90mL of deionized water. This resulting 100mL solution is sufficient to make a gel
Step 1: Add 0.8 g of agarose in 100mL of 1X buffer to a 250mL beaker. Heat and stir until the solution is clear. Allow to cool to 55 degrees Celsius.
Step 2: Ready agarose gels can be stored at room temperature. Prior to electrophoresis, place in a microwave oven for 1 minute until completely liquefied.
DNA labeling solution
Dissolve 10 mL of the concentrated label solution in 90 mL of hot tap water solution.
DNA extraction from cells and labeling
Step 1: Place 10mL of dissolved onion cell solution in a test tube.
Step 2: Hold the test tube at a 45 degree angle and slowly add 5-7mL of frozen ethanol. Ethanol will form a second layer on top of the solution. Leave the solution for 2-3 minutes. You will see DNA floating in the alcohol layer.
Step 3: Bring the DNA vaccination tube to the interface of the two layers and stir.
Step 4: Carefully lift the inoculum tube and place the DNA sample on the rim of the test tube. when DNA is stretched it forms thin threads,
This Activity aims at assembling the electrophoresis device, forming wells and finally electrophoresis and DNA observation.
The technique of electrophoresis involves the separation and movement of DNA molecules in a gel which is exposed to an electric field. DNA is a negatively charged molecule and so the DNA fragments move towards the positive pole. Small fragments move faster than large ones.