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Home Κατάστημα Laboratory Equipment Chemical Reagents Indicators Phenolphthalein 100g
Paraffin wax mp 58-60 °C in pellets 500g €12,90 €16,00 με ΦΠΑ
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Lugol’s Iodine 5% Solution 125ml €20,16 €25,00 με ΦΠΑ

Phenolphthalein 100g

€20,16 €25,00 με ΦΠΑ

Phenolphthalein 100g
3,3-Bis(4-hydroxyphenyl)-2-benzofuran-1(3H)-one
C20H14O4
MW: 318.328 g·mol−1
https://pubchem.ncbi.nlm.nih.gov/compound/4764

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SKU: 330792 Categories: Chemical Reagents, Chemical Reagents, Indicators, Indicators
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Phenolphthalein 100g

Phenolphthalein 100g
3,3-Bis(4-hydroxyphenyl)-2-benzofuran-1(3H)-one
C20H14O4
MW: 318.328 g·mol−1
https://pubchem.ncbi.nlm.nih.gov/compound/4764

Phenolphthalein is a chemical compound with the formula C20H14O4 and is often written as “HIn“, “HPh“, “phph” or simply “Ph” in shorthand notation. Phenolphthalein is often used as an indicator in acid–base titrations. For this application, it turns colorless in acidic solutions and pink in basic solutions. It belongs to the class of dyes known as phthalein dyes.

Phenolphthalein is slightly soluble in water and usually is dissolved in alcohols for use in experiments. It is a weak acid, which can lose H+ ions in solution. The nonionized phenolphthalein molecule is colorless and the double deprotonated phenolphthalein ion is fuchsia. Further loss of proton in higher pH occurs slowly and leads to a colorless form. Phenolphthalein ion in concentrated sulfuric acid is orange red due to sulfonation.

Uses

pH indicator

Phenolphthalein’s common use is as an indicator in acid-base titrations. It also serves as a component of universal indicator, together with methyl red, bromothymol blue, and thymol blue.

Phenolphthalein adopts different forms in aqueous solution depending on the pH of the solution. Inconsistency exists in the literature with regard to the hydrated forms of the compounds and the color in sulfuric acid. Wittke reported in 1983 that under strongly acidic conditions, it exists in protonated form (HIn+), providing an orange coloration. However, later paper suggested that this color is due to sulfonation to phenolsulfonphthalein.

Between strongly acidic and slightly basic conditions, the lactone form (HIn) is colorless. The doubly deprotonated (In2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH)3− form, and its pink color undergoes a rather slow fading reaction and becomes completely colorless when pH is greater than 13.

The pKA values of phenolphthalein was found to be 9.05, 9.50 and 12 while that of phenolsulfonphthalein are 1.2 and 7.70.

Species H3In+ H2In In2− In(OH)3−
Structure Phenolphthalein-very-low-pH-2D-skeletal.svg Phenolphthalein-low-pH-2D-skeletal.svg Phenolphthalein-mid-pH-2D-skeletal.svg Phenolphthalein-high-pH-2D-skeletal.svg
Model Phenolphthalein-orange-very-low-pH-3D-balls.png Phenolphthalein-colourless-low-pH-3D-balls.png Phenolphthalein-red-mid-pH-3D-balls.png Phenolphthalein-colourless-high-pH-3D-balls.png
pH <-1 in H2SO4[4] 0−8.3 8.3−10.0[7] >10.0
Conditions strongly acidic acidic or near-neutral basic strongly basic
Color orange colorless pink to fuchsia colorless
Image Phenolphthalein-in-conc-sulfuric-acid.jpg Phenolphthalein-at-pH-9.jpg
PP startAnimGif
An animation of the pH dependent reaction mechanism: H3In+ → H2In → In2− → In(OH)3−

Medical uses

Phenolphthalein has been used for over a century as a laxative, but is now being removed from over-the-counter laxatives because of concerns over carcinogenicity. Laxative products formerly containing phenolphalein have often been reformulated to have alternate active ingredients: Feen-a-Mint switched to bisacodyl and Ex-Lax  was switched to a senna extract.

Thymolphthalein is a related laxative made from thymol.

Despite concerns regarding its carcinogenicity, the use of phenolphthalein as a laxative is unlikely to cause ovarian cancer. Phenolphthalein has been found to inhibit human cellular calcium influx via store-operated calcium entry (SOCE, see Calcium release activated channel § Structure). This is effected by its inhibiting thrombin and thapsigargin, two activators of SOCE that increase intracellular free calcium.

Phenolphthalein has been added to the European Chemicals Agency‘s candidate list for Substances of Very High Concern (SVHC).

A reduced form of phenolphthalein, phenolphthalin, which is colorless, is used in a test to identify substances thought to contain blood, commonly known as the Kastle–Meyer test. A dry sample is collected with a swab or filter paper. A few drops of alcohol, then a few drops of phenolphthalin, and finally a few drops of hydrogen peroxide are dripped onto the sample. If the sample contains hemoglobin, it will turn pink immediately upon addition of the peroxide, because of the generation of phenolphthalein.
A positive test indicates the sample contains hemoglobin and, therefore, is likely blood. A false positive can result from the presence of substances with catalytic activity similar to hemoglobin. This test is not destructive to the sample; it can be kept and used in further tests. This test has the same reaction with blood from any animal whose blood contains hemoglobin, including almost all vertebrates; further testing would be required to determine whether it originated from a human.

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